The migration potential of A375 cells was hampered by the OB extract in a concentration-dependent manner. In the set of experimental conditions, the OB extract only manifested significant antiproliferative and pro-apoptotic activity against the A375 human melanoma cell line at the highest tested concentration, namely 60 μg/mL. It was bactericidal only against Candida spp. The tested extract had a mild bacteriostatic effect on the tested bacterial strains. The total phenolic content was 631.496 µgGAE/mL of extract and the antioxidant activity was 7258.67 μmolTrolox/g of extract. The main polyphenols and flavonoids identified were gallic acid, caffeic acid, epicatechin, coumaric acid, ferulic acid, rutin and rosmarinic acid. The aim of this study was to evaluate the phytochemical composition and biological activity of OB hydroalcoholic extract and to provide directions for the antimicrobial effect, antiproliferative and pro-apoptotic potential against A375 melanoma cell line, and anti-angiogenic and anti-inflammatory capacity. In therapy, the most frequently used type of extracts are from the aerial part, which are the fatty oils obtained from the seeds and have a wide range of medicinal properties. (OB), also commonly known as evening primrose, belongs to the Onagraceae family and has the best studied biological activity of all the members in the family. 7 Department of Pharmaceutical Chemistry, University of Medicine and Pharmacy 'Victor Babeş', Eftimie Murgu Square, No.6 Department of Toxicology, University of Medicine and Pharmacy 'Victor Babeş', Eftimie Murgu Square, No.5 Department of Pharmaceutical Physics, University of Medicine and Pharmacy 'Victor Babeş', Eftimie Murgu Square, No.4 Department of Microbiology, University of Medicine and Pharmacy 'Victor Babeş', Eftimie Murgu Square, No.3 Department of Food Control, Banat's University of Agricultural Sciences and Veterinary Medicine 'King Michael I of Romania' from Timişoara, Calea Aradului No.

2 Department of Pharmacology and Clinical Pharmacy, University of Medicine and Pharmacy 'Victor Babeş', Eftimie Murgu Square, No.1 Department of Pharmacognosy, University of Medicine and Pharmacy 'Victor Babeş', Eftimie Murgu Square, No.

Oenothera biennis lunar moth